تاثیر سه ماه تمرین مقاومتی، سه ماه بی‌تمرینی، و سه ماه بازتمرینی بر سطوح سرمی IL-8 مردان میانسال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد فیزیولوژی ورزشی، گروه تربیت بدنی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، لرستان، ایران.

2 استاد فیزیولوژی ورزشی، گروه تربیت‌‌بدنی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، لرستان، ایران

3 استادیار، فیزیولوژی ورزشی، گروه تربیت بدنی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، لرستان، ایران.

10.22091/arsnes.2024.10507.1009

چکیده

هدف: هدف پژوهش حاضر، بررسی تاثیر سه ماه تمرین مقاومتی، بی‌‌تمرینی، و بازتمرینی بر سطوح سرمی IL-8 مردان میانسال می‌‌باشد.
روش‌‌: در این مطالعه تعداد 40 مرد میانسال (30 الی 50 سال) واجد شرایط به‌‌صورت تصادفی انتخاب شدند و براساس شاخص‌‌های آنتروپومتریکی و یک تکرار بیشینه حرکات تمرینی، در دو گروه مداخله (EXE) (20 نفر) و کنترل (CTR)
(20 نفر) قرار گرفتند. هر دو گروه به‌طور متوالی یک دوره تمرین (12 هفته)، بی‌‌تمرینی (12 هفته) و یک دوره بازتمرینی
(12 هفته) را زیر نظر پژوهشگر انجام دادند. برای بررسی نتایج از آزمون‌‌های کولموگروف اسمیرنف و لون برای بررسی نرمال بودن داده‌‌ها و همگنی واریانس‌‌ها استفاده شد. همچنین برای مقایسه گروه‌ها در متغیرهای مورد مطالعه از آزمون واریانس با اندازه‌‌گیری‌‌های مکرر (Repeated Measure) در سطح معنی‌داری 0.05>P استفاده گردید.
یافته‌ها: براساس نتایج به‌‌دست آمده از آزمون تحلیل واریانس با اندازه‌‌گیری‌‌های مکرر، 12 هفته تمرین مقاومتی باعث افزایش سطوح سرمی IL-8 در مردان میانسال و دوره بی‌‌تمرینی (سه ماه) باعث برگشت این سطوح به حالت اولیه و 12 هفته بازتمرینی باعث افزایش بیشتر این سطوح نسبت به دوره تمرین اولیه شد.
نتیجه‌‌گیری: IL-8 در سازگاری‌‌های عضلانی ناشی از تمرین مقاومتی در پدیده حافظه عضلانی درگیر است، و به عنوان یک راهکار برای بهبود شرایط فیزیولوژیکی، افزایش سنتز پروتئین و همچنین افزایش قدرت، حجم و عملکرد توده عضلانی در افراد میانسال، در نظر گرفته شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Impact of Three-Month Resistance Training, Three-Month Non-Training, and Three-Month Retraining on the Serum Levels of IL-8 in Middle-Aged Men

نویسندگان [English]

  • Hadis Mehrabifard 1
  • Masoud Rahmati 2
  • Rahim Mirnasouri 3
1 Master's Degree in Exercise Physiology, Department of Physical Education, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran
2 Professor of Exercise Physiology, Department of Physical Education, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran
3 Assistant Professor, Sports Physiology, Department of Physical Education, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran
چکیده [English]

Purpose: The study aims to examine the impact of three months of resistance training,
non-training, and retraining on the serum levels of IL-8 in middle-aged men.
Method: In this study, 40 eligible middle-aged men (aged 30 to 50 years) were randomly selected. They were divided into two groups based on anthropometric indicators and maximum repetition of exercise movements: an intervention group (EXE) consisting of 20 individuals, and a control group (CTR) also consisting of 20 individuals. Both groups underwent a training period
(12 weeks), a non-training period (12 weeks), and a retraining period (12 weeks) under the researcher's supervision. Kolmogorov-Smirnov and Lilliefors tests were used to assess data normality and variance homogeneity. The repeated measures analysis of variance test was employed to compare the groups on the studied variables, with a significance level set at P>0.05.
Findings: Based on the results obtained from the analysis of variance test with repeated measurements, 12 weeks of resistance training increases serum levels of IL-8 in middle-aged men. A non-training period (three months) causes these levels to return to their original state. Furthermore, 12 weeks of retraining increased these levels more than the initial training period.
Conclusion: IL-8 is involved in muscle adaptations caused by resistance training in the phenomenon of muscle memory. As a strategy for improving physiological conditions, increasing protein synthesis, and enhancing the strength, volume, and function of muscle mass in middle-aged individuals, it should be considered.

کلیدواژه‌ها [English]

  • Resistance training
  • Non-training
  • Retraining
  • IL-8 serum levels
  • Middle-aged men
  1. Sharples AP & Turner DC. Skeletal muscle memory. American Journal of Physiology-Cell Physiology. 2023; 324(6): C1274-C94.
  2. Sharples AP, Stewart CE & Seaborne RA. Does skeletal muscle have an ‘epi’‐memory? The role of epigenetics in nutritional programming, metabolic disease, aging and exercise. Aging cell. 2016; 15(4): 603-16.
  3. Seaborne RA, Strauss J, Cocks M, Shepherd S, O’Brien TD, Van Someren KA & et al. Human skeletal muscle possesses an epigenetic memory of hypertrophy. Scientific reports. 2018; 8(1).
  4. Staron RS, Leonardi MJ, Karapondo DL, Malicky ES, Falkel JE, Hagerman FC & Hikida RS. Strength and skeletal muscle adaptations in heavy-resistance-trained women after detraining and retraining. Journal of applied physiology. 1991; 70(2): 631-40.
  5. Bruusgaard JC, Egner IM, Larsen TK, Dupre-Aucouturier S, Desplanches D & Gundersen K. No change in myonuclear number during muscle unloading and reloading. Journal of applied physiology. 2012; 113(2): 290-6.
  6. Yoshimura T, Matsushima K, Tanaka S, Robinson EA, Appella E, Oppenheim JJ & Leonard AJ. Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defese cytokines. Journal of Immunology. 2019; 202(1): 5-9.
  7. Matsushima K, Yang D & Oppenheim Interleukin-8: An evolving chemokine. Cytokine. 2022; 153: 155828.
  8. Ancrile BB, O’Hayer KM & Counter CM. Oncogenic Ras–Induced Expression of Cytokines in Cancer. Molecular interventions. 2008; 8(1): 22.
  9. Bruun JM, Pedersen SB, Kristensen K & Richelsen B. Opposite regulation of Interleukin‐8 and tumor necrosis factor‐α by weight loss. Obesity Research. 2002; 10(6): 499-506.
  10. Chan MS, Carey AL, Watt MJ & Febbraio MA. Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2004; 287(2): R322-R7.
  11. Boekholdt SM, Peters RJ, Hack CE, Day NE, Luben R, Bingham SA & et al. IL-8 plasma concentrations and the risk of future coronary artery disease in apparently healthy men and women: the EPIC-Norfolk prospective population study. Arteriosclerosis, thrombosis, and vascular biology. 2004; 24(8): 1503-8.
  12. Hou Y-C, Wang C-J, Chao Y-J, Chen H-Y, Wang H-C, Tung H-L & et al. Elevated serum interleukin-8 level correlates with cancer-related cachexia and sarcopenia: an indicator for pancreatic cancer outcomes. Journal of clinical medicine. 2018; 7(12): 502.
  13. Callaway CS, Delitto AE, D’Lugos AC, Patel R, Nosacka RL, Delitto D & et al. IL-8 released from human pancreatic cancer and tumor-associated stromal cells signals through a CXCR2-ERK1/2 axis to induce muscle atrophy. 2019; 11(12): 1863.
  14. Santos JDMBd, Bachi ALL, Luna Junior LA, Foster R, Sierra APR, Benetti M & et al. The relationship of IL-8 and IL-10 myokines and performance in male marathon runners presenting exercise-induced bronchoconstriction. International Journal of Environmental Research and Public Health. 2020; 17(8): 2622.
  15. Seaborne RA, Strauss J, Cocks M, Shepherd S, O’Brien TD, Van Someren KA & et al. Human skeletal muscle possesses an epigenetic memory of hypertrophy. Scientific reports. 2018; 8(1): 1898.
  16. Petersen EW, Ostrowski K, Ibfelt T, Richelle M, Offord E, Halkjær-Kristensen J & et al. Effect of vitamin supplementation on cytokine response and on muscle damage after strenuous exercise. American Journal of Physiology-Cell Physiology. 2001; 280(6): C1570-C5.
  17. Cho M-R, Lee S & Song S-K. A review of sarcopenia pathophysiology, diagnosis, treatment and future direction. Journal of Korean Medical Science. 2022; 37(18).
  18. Blocquiaux S, Gorski T, Van Roie E, Ramaekers M, Van Thienen R, Nielens H & et al. The effect of resistance training, detraining and retraining on muscle strength and power, myofibre size, satellite cells and myonuclei in older men. Experimental gerontology. 2020; 133: 110860.‏
  19. Sugawara K, Takahashi H, Kasai C, Kiyokawa N, Watanabe T, Fujii S & et al. Effects of nutritional supplementation combined with low-intensity exercise in malnourished patients with COPD. Respiratory medicine. 2010; 104(12): 1883-9.
  20. Cruz LGd, Zanetti HR, Andaki ACR, Mota GRD, Barbosa Neto O & Mendes EL. Intradialytic aerobic training improves inflammatory markers in patients with chronic kidney disease: a randomized clinical trial. Motriz: Revista de Educação Física. 2018; 24(03).
    https://doi.org/10.1590/s1980-657420180003e017517
  21. Nielsen AR & Pedersen BK. The biological roles of exercise-induced cytokines: IL-6, IL-8, and IL-15. Applied physiology, nutrition, and metabolism. 2007; 32(5): 833-9.
  22. Petersen EW, Ostrowski K, Ibfelt T, Richelle M, Offord E, Halkjær-Kristensen J & et al. Effect of vitamin supplementation on cytokine response and on muscle damage after strenuous exercise. American Journal of Physiology-Cell Physiology. 2001; 280(6): C1570-C5.
  23. Semenza GL, Shimoda LA & Prabhakar NR. Regulation of gene expression by HIF‐1. Novartis Foundation Symposium. 2006; 272: 2-8; discussion 8-14, 33-6.
    https://doi.org/1002/9780470035009.ch2
  24. Dogra S, Wolf M, Jeffrey MP, Foley RC, Logan-Sprenger H, Jones-Taggart H & et al. Disrupting prolonged sitting reduces IL-8 and lower leg swell in active young adults. BMC Sports Science, Medicine and Rehabilitation. 2019; 11: 1-7. https://doi.org/10.1186/s13102-019-0138-4
  25. Turoń-Skrzypińska A, Rotter I, Przybyciński J, Szylińska A, Mińko A, Ciechanowski K & et al. Does Exercising with the Use of Virtual Reality during Haemodialysis Have an Impact on Plasma Levels of Interleukin 1β, Interleukin 6, and Interleukin 8? Journal of Clinical Medicine. 2023; 12(16): 5358.
  26. Kolaczkowska E & Kubes P. Neutrophil recruitment and function in health and inflammation. Nat Rev Immunol. 2013; 13(3): 159-75. https://doi.org/10.1038/nri3399
  27. Kraft R, Herndon DN, Finnerty CC, Cox RA, Song J & Jeschke MG. Predictive value of IL-8 for sepsis and severe infections after burn injury: a clinical study. 2015; 43(3): 222–7.
  28. Henkels KM, Frondorf K, Gonzalez-Mejia M E, Doseff A L & Gomez-Cambronero IL-8-induced neutrophil chemotaxis is mediated by Janus kinase 3 (JAK3). FEBS letters. 2011; 585(1): 159-66. https://doi.org/10.1016/j.febslet.2010.11.031
  29. Qazi BS, Tang K & Qazi Recent advances in underlying pathologies provide insight into interleukin-8 expression-mediated inflammation and angiogenesis. International journal of inflammation. ‏2011. 908468. https://doi.org/10.4061/2011/908468
  30. Koch AE, Polverini PJ, Kunkel SL, Harlow LA, DiPietro LA, Elner VM & et al. Interleukin-8 as a macrophage-derived mediator of angiogenesis. 1992; 258(5089): 1798-801.
    https://doi.org/10.1126/science.1281554
  31. Puglisi MJ & Fernandez ML. Modulation of C-reactive protein, tumor necrosis factor-α, and adiponectin by diet, exercise, and weight loss. The Journal of nutrition. 2008; 138(12): 2293-6. https://doi.org/10.3945/jn.108.097188
  32. Chen G-Q, Mou C-Y, Yang Y-Q, Wang S & Zhao Z-W. Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes. Life sciences. 2011; 89(1-2): 44-9. https://doi.org/10.1016/j.lfs.2011.04.018