The Effect of high intensity interval training on serum levels of protein-D surfactant and insulin resistance index in healthy and diabetic rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sports Sciences, University of Qom, Qom, Iran.

2 Associate Professor, Department of Sports Physiology and Pathology, Faculty of Sport Sciences; Institute of Applied Studies in Sport Sciences, Arak University, Arak, Iran

3 PhD student in exercise physiology, Faculty of Sport Sciences, University of Tehran, Tehran, Iran

10.22091/arsnes.2025.14210.1038

Abstract

Background: The aim of present study effect of high intensity interval training (HIIT) on serum levels of protein-D surfactant (SPD) and insulin resistance index in healthy and type 2 diabetic rats.
Methods: In this experimental study, Forty- six mature male Albino Wistar rats whit weighing 241.92±23.03 gr, randomly divided in to four groups: healthy control, diabetic control, diabetic HIIT and healthy HIIT. For induction of diabetes, was used intraperitoneal injection of nicotinamide solution (120 mg/kg) and STZ solution (65 mg/kg). HIIT protocol was performed for 10 weeks and animals were dissected 24 hours after the last session and blood serum of rats was collected for analysis. Data were analyzed using one-way ANOVA and LSD’s Post Hoc test at 0.05%. Results: HIIT caused a significant decrease in blood glucose in diabetic HIIT group compared to the diabetic control group (P=0.00). Serum levels of protein-D surfactant (SPD) in the diabetic control group significantly decreased (P=0.01) and the insulin resistance index (P=0.00) was significantly higher than healthy controls. 10 weeks of HIIT training significantly increased in SPD (p=0.00) in diabetic HIIT group compared to diabetic control group. Also, HIIT significantly decreased insulin resistance index (p= 0.00) and insulin (p=0.00) in diabetic HIIT group compared to diabetic control group.
Conclusion: It seems that HIIT training by increasing the levels of serum SPD improve insulin resistance index in type 2 diabetic rat.

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History

  • Receive Date: 13 July 2025
  • Revise Date: 23 July 2025
  • Accept Date: 27 August 2025
  • First Publish Date: 23 September 2025
  • Publish Date: 23 September 2025

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