The Impact of Long-Term Endurance Training on Testicular Tissue Oxidative Stress in Adult Male Rats with Ionizing Radiation-Induced Infertility

Document Type : Original Article

Authors

1 Associate Professor, Department of Sports Physiology and Pathology, Faculty of Sport Sciences; Institute of Applied Studies in Sport Sciences, Arak University, Arak, Iran

2 MSc, Department of Sports Physiology and Pathology, Faculty of Sport Sciences, Arak University, Arak, Iran

10.22091/arsnes.2024.11192.1014

Abstract

Objective: Radiotherapy is the most common method for treating cancer. Testicular tissue damage caused by radiotherapy appears to be primarily due to oxidative stress, which results
from an excessive production of reactive oxygen and nitrogen species, coupled with decreased antioxidant levels. Therefore, short-term, moderate-intensity exercise can positively impact the structure of testicular tissue and enhance antioxidant status.
Method: In this study, we aimed to investigate the effects of continuous moderate-intensity endurance training on serum malondialdehyde levels, total antioxidant capacity, and testicular tissue structure in male rats exposed to ionizing X-rays. A total of 24 male Sprague-Dawley rats, with an average weight of 221 grams, were divided into four groups: a healthy control group, a healthy endurance training group, a control group subjected to irradiation, and an endurance training group exposed to irradiation. After eight weeks of endurance training, the rats were dissected, and the testicular tissue was isolated for stereological studies. Additionally, blood serum was collected to measure oxidant and antioxidant factors. The data were analyzed using a one-way analysis of variance in SPSS software.
Findings: After 8 weeks of endurance training, a significant difference was observed in serum malondialdehyde levels among the studied groups (P=0.001). Additionally, a significant difference was noted in the total antioxidant capacity across the four groups (P=0.001). Regarding testicular structure, several factors were found to be significantly different, including estimated shrinkage
(P=0.001), seminiferous tubule volume (P=0.001), seminiferous tubule length (P=0.016), and seminiferous tubule diameter (P=0.001).
Conclusion: The conclusion drawn from this study is that eight weeks of moderate-intensity endurance training significantly impacted serum levels of malondialdehyde and total antioxidant capacity, as well as the testicular structure of male rats undergoing radiotherapy.

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References

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Applied Research in Sports Nutrition and Exercise Science
Volume 1, Issue 3
December 2024
Pages 43-58

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History

  • Receive Date: 16 August 2024
  • Revise Date: 09 September 2024
  • Accept Date: 21 October 2024
  • First Publish Date: 22 October 2024
  • Publish Date: 22 October 2024

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